The effect of TRIM5αon the proliferation and apoptosis of hepatocellular carcinoma cells / 实用肿瘤学杂志

ABSTRACT

Ob jective To study the effect of Tripartite motif 5 alpha( TRIM5α) on the proliferation and apoptosis of hepatocellular carcinoma cells.Methods The TRIM5α construction was identified by enzyme diges-tion,PCR and sequencing.The TRIM5αplasmid was transfected into HepG2 cells and identified by RT-PCR and Western blotT.he cell proliferation was monitored by RTCA real -time instrument and cell apoptosis was an-alyzed by flow cytometry.The apoptosis related proteins were detected by Western blot.Results The TRIM5αvector was successfully constructed.The result of RT-PCR and Western blot showed that TRIM5αplasmid could enter HepG2 cells.TRIM5αcould inhibit HepG2 cells proliferation also increased their apoptosis through down-regulation of Bcl-2 expression and activation of Caspase-3 expression.TRIM5αalso could inhibit the tumor formation of HepG2 cells in vivo.Conclusion TRIM5αinhibited cell proliferation, promoted apoptosis and weakened the tumorigenic ability of HepG2 cells.This will establish the foundation for the mechanism study and the clinic use of TRIM5αfor tumor therapy in the future.