Restorative effect of (5E, 13E)-5,13-Docosadienoic acid on carbon tetrachloride induced oxidative stress in rats

ABSTRACT

Objective: To evaluate the restorative effect of (5E, 13E)-5,13-Docosadienoic acid on carbon tetrachloride induced oxidative stress in rats. Methods: Wistar strain male albino rats, weighing 180-200 g/bw were selected for the study. Rats were divided into four groups. Group I animals were served as normal control. Group II was administered with corn oil (3 ml/kg, i.p.) as vehicle control. Group III was given single dose (29th day) of CCl4 in corn oil (11 v/v, 3 ml/kg, i.p.). Groups IV was treated with (5E, 13E)-5,13-Docosadienoic acid (DA) (6 mg/kg body weight) for 28 days and given single dose of (29th day) CCl4 in corn oil (11 v/v, 3 ml/kg, i.p.). Six hours after CCl4 intoxication, the experimental animals were sacrificed. The blood samples were collected. Liver was excised immediately and immersed in physiological saline. Results: The lipid peroxidation was initiated in CCl4 intoxicated rats which is evidenced by thiobarbituric acid (TBARS) and diminution of GSH content in liver. Super oxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), vitamin C and E in CCl4 intoxicated rats retrieved towards near normalcy. After treating with DA which significantly altered (P<0.001) serum marker enzyme level and antioxidant level near normal against CCl4 treated rats. Conclusions: It was observed that the entire variable tested i.e., SOD, CAT, GPx, reduced glutathione, vitamin C and E recorded a significant decline on CCl4 treatment. However, treatment with DA restored the levels to near normal value, suggesting the therapeutic effect of DA to counter the oxidative stress.