Morpho-anatomy, physicochemical and phytochemical standardization with HPTLC fingerprinting of aerial parts of Rivea hypocrateriformis

ABSTRACT

Objectives: To study the morpho-anatomy of the aerial parts of Rivea hypocrateriformi (Convolvulaceae) to increase the knowledge and standardization parameters of these plants and its family. Methods: Morpho-anatomical studies of aerial parts of have been carried out by free hand. The different types of histochemical tests were performed for the identification of micro-chemical by using staining reagents. Preliminary phytochemical and quantitative estimation has been determined along with HPTLC fingerprinting. CAMAG HPTLC system equipped with Linomat V applicator, TLC scanner 3, Reprostar 3 and WIN CATS-4 software was used for fingerprinting. Results: The morpho-anatomical structure of plant revealed the presence of thick wall collenchyma cell and parenchyma cells along with spharaphides and columnar palisade cells. Presense of numerous covering trichomes, parasitic stomata, oval shaped vascular bundles and calcium oxalate crystals were observed. In powder microscopy lignified xylem vessels, and covering trichomes were clearly seen. Physicochemical parameters, ash value, inorganic elements, moisture content and extractive value were determined to develop the stringent pharmacognostic standards. Qualitative standardization and HPTLC fingerprinting of phytochemical revealed the presense of various plant secondary metabolites. Conclusions:These findings will be useful in establishing pharmacognostic and phytochemical standards for identification, as well as assessment of purity and quality of this plant, which definitely gaining the relevance in plant drug research and establishment of plant monograph.