Optimization of Microwave-assisted Extraction of Polyphenols from Enteromorpha prolifra by Orthogonal Test / 中草药·英文版

ABSTRACT

Objective To optimize microwave-assisted extraction of polyphenols from Enteromorpha prolifra.Methods Based on single-factor tests,an efficient microwave-assisted extraction(MAE)technique was developed to extract bioactive polyphenols from E.prolifra through orthogonal L16(4)5 test.Results The highest yield(0.923±0.013)mg/g was obtained when microwave power,solvent to raw material ratio,irradiation time,ethanol concentration,and extraction cycles were 500 W,25 mL/g,25 min,40%,and 3,respectively,which was higher than that of Soxhlet extraction with methanol for 6 h,ultrasound-assisted extraction with 40% ethanol for 1 h twice and heat reflux extraction with 40%ethanol for 2 h twice.Conclusion This finding indicates that MAE is a superior technique for the extraction of polyphenols due to less impurity,higher time efficiency and yield.