Simultaneous Determination of Seven Flavonoids in Aerial Parts of Artemisia frigida by HPLC / 中草药·英文版
Chinese Herbal Medicines
;
(4): 252-258, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-499810
ABSTRACT
Objective To establish an HPLC method for the determination of seven flavonoids from the aerial part of Artemisia frigida.Methods Hypersil ODS-2 (300 mm× 4.6 mm,5 μm) column was used,with acetonitril-0.2% phosphoric acid (gradient elution) as a mobile phase,and the detection wavelength was at 283 nm with flow rate at 1mL/min.Results All calibration curves showed good linear regression (r > 0.9990) within the tested range.All average recovery was more than 98.00% and RSD was less than 3.0% (n =6).Conclusion The method is steady and with good repeatability,and could be used to determine the content of flavonoids in A.frigida from different areas.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Herbal Medicines
Year:
2012
Type:
Article
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