Establishment of a rapid method for the isolation of murine peritoneal macrophages / 局解手术学杂志
Journal of Regional Anatomy and Operative Surgery
; (6): 130-131, 2015.
Article
in Zh
| WPRIM
| ID: wpr-499900
Responsible library:
WPRO
ABSTRACT
Objective To establish a rapid method for the isolation of murine peritoneal macrophages. Methods Abdominal lavage was performed with NS and the peritoneal macrophages was purified from the lavage,which was later transferred into high glucose DMEM cul-ture medium. Cell viability was measured via the typan blue staining. Purity was observed via Wright's stain. Results High purity macropha-ges with typical morphology were obtained. Conclusion A simple and realistic method was set up for the isolation of murine peritoneal mac-rophages.
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WPRIM
Language:
Zh
Journal:
Journal of Regional Anatomy and Operative Surgery
Year:
2015
Type:
Article