Effect of tumor necrosis factor-alpha preconditioning and ischemic preconditioning on hepatic ischemia-reperfu-sion injury in rats / 局解手术学杂志

ABSTRACT

Objective To compare the effect of TNF-α preconditioning and ischemic preconditioning on hepatic ischemia-reperfusion injury ( IRI) and investigate the underlying mechanisms of TNF-αpreconditioning. Methods Fourty healthy male Wistar rats were random-ly divided into four groups which were Sham-operated group ( SO) ,ischemia-reperfusion group ( IR groupproduced by total inflow occlusion for 30 min) ,ischemic preconditioning group ( IPC groupinduce with 10 min hepatic ischemic and open 10 min before IR) and TNF-αpre-conditioning group ( TPC groupintraperitoneal injection with 1 μg/kg TNF-a 30 min prior to IR) . The sample of blood and hepatic tissue of all groups were taken after experiment. The protein levels of NF-κBp65 and Bcl-2 in the hepatic tissue were detected by immunohistochemis-try. Results There was significant difference (P0. 05) between IPC group and TPC group. Conclusion TNF-α preconditioning decreased the intensity of hepatic IRI,just as ischemic preconditioning,by induces an de-crease in the NF-κBp65 expression and an increase in the Bcl-2 expression.