Apoptosis in Lungs and Liver after Crush Injury of Hindlimbs in Rat / 法医学杂志
Journal of Forensic Medicine
;
(6): 88-92, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-500282
ABSTRACT
Objective To investigate the process of apoptosis in lungs and liver induced by crushing hindlimbs of rat, and study the mechanismof crush injury. Methods The rat experimental model of hindlimbs crush injury was established. The cell apoptosis in lungs and liver was detected by TUNEL assay, and the expression of Bax, Bcl-2 and caspase-3 apoptin was examined by immunohistochemistry. Results C ompared with the control group, the partial muscle injury of rat’s hindlimbs was more serious with more apoptosis observed in lungs and liver (P<0.05). The expression of Bax was up-regulated and Bcl-2 was down-regulated, whereas caspase-3 expression was activated (P<0.05). Conclusion The cell apoptosis has increased significantly in lungs and liver after crush injury of hindlimbs in rat. The corre-lation factor released during tissue injury may mediate apoptosis process.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Prognostic study
Language:
Chinese
Journal:
Journal of Forensic Medicine
Year:
2015
Type:
Article
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