Content Determination of 4 Ingredients in Ligustrum lucidum Decoration Piece by Reference Extract Method / 中国药房
China Pharmacy
;
(12): 4685-4687, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-500857
ABSTRACT
OBJECTIVE:
To establish a method for the contents determination of 4 ingredients in Ligustrum lucidum decoration piece by reference extract method.METHODS:
HPLC was performed on the column of XBridge C18 with mobile phase of acetoni-trile-0.1% formic acid(gradient elution) at flow rate of 1.0 ml/min,detection wavelength was 254 nm,column temperature was 30℃and volume injection was 10μl.RESULTS:
The linear range was 0.037-0.598 mg for neonuezhenide,0.006-0.101 mg for ac-teoside,0.189-3.023 mg for nuezhenide and 0.314-5.027 mg for specnuezhenide(r≥0.999 0);RSDs of precision,reproducibility and stability tests were no more than 3.8%;recoveries were 98.46%-104.83%(RSD=2.43,n=6),95.55%-104.57%(RSD=3.63, n=6),100.09%-104.39%(RSD=1.45,n=6)and 98.84%-104.97%(RSD=2.02,n=6).CONCLUSIONS:
The method is simple, good reproducibility,and can be used for the contents determination of 4 ingredients in L. lucidum decoration piece.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2015
Type:
Article
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