Content Determination of Gallic Acid in Different Preparation Parts of Phyllanthus emblica by HPLC / 中国药房

ABSTRACT

OBJECTIVE:To establish a method for the content determination of gallic acid in different preparation parts of Phyl-lanthus emblica. METHODS:HPLC was performed on the column of ZORBAX Extend C18 with mobile phase of methanol-0.1%phosphoric acid (1090,V/V) at flow rate of 1.0 ml/min,detection wavelength was 270 nm,column temperature was 30 ℃ and the volume injection was 10 μl. RESULTS:The linear range of gallic acid was 0.042 5-0.212 5 mg/ml;RSDs of precision,accura-cy and stability tests were lower than 3.0%;recovery was 99.38%-102.14%(RSD=1.045,n=6). The mass fraction of gallic acid in P. emblica was 1.80%,and the content of gallic acid in different preparation parts was 0.70%-2.38%. CONCLUSIONS:The method is simple,reproducibility,and can be used for the content determination of gallic acid in different preparation parts of P. em-blica.