Simultaneous Determination of the Content of 4 Psoralen Compounds in Buwu Tincture by HPLC / 中国药房
China Pharmacy
;
(12): 4734-4736, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-500880
ABSTRACT
OBJECTIVE:
To establish a method for simultaneous determination of 4 psoralen compounds in Buwu tincture.METHODS:
HPLC was performed on the column of Dikma Diamonsil C18 with mobile phase of acetonitrile-0.2% Acetic acid by gradient elution at flow rate of 1 ml/min,detection wavelength was 246 nm,column temperature was 30 ℃,and the injection vol-ume was 15 μl.RESULTS:
The linear range was 13.00-208 μg/ml for angelicin,26.00-416 μg/ml for bavachin,24.50-392 μg/ml for psoralidin and 37.88-606 μg/ml for isobavachalcone,respectively(r≥0.999 6);RSDs of precision,stability and reproducibility tests were less than 2.00%;recoveries were 95.22%-97.23%(RSD=0.87%,n=6),100.24%-104.64%(RSD=1.62%,n=6), 102.28%-104.39%(RSD=1.47%,n=6)and 97.68%-100.17%(RSD=0.97%,n=6),respectively.CONCLUSIONS:
The method is simple and reproducible,and can be used for the quality control of Buwu tincture.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2015
Type:
Article
Similar
MEDLINE
...
LILACS
LIS