Simultaneous Contents Determination of Tetrahydropalmatine and Rosmarinic Acid in Xiaoheling Capsule by HPLC / 中国药房
China Pharmacy
;
(12): 4719-4721, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-500886
ABSTRACT
OBJECTIVE:
To establish a method for the contents determination of tetrahydropalmatine and rosmarinic acid in Xiaoheling capsule.METHODS:
HPLC was performed on the column of Phenomenex C18 with mobile phase A of 0.1% phosphoric acid(adjusted pH to 6.0 with triethylamine)-methanol(4555,V/V) and (adjusted pH to 6.0 with triethylamine) B of 0.1% phos-phoric acid-methanol(5545,V/V)(gradient elution)at flow rate of 0.8 ml/min,detection wavelength was 282 nm(0-25 min)and 330 nm(25.01-60 min)and volume size was 10 μl.RESULTS:
The linear range was 1.502-75.10 μg/ml for tetrahydropalmatine and 6.03-301.6 μg/ml for rosmarinic acid(r=0.999 8);RSDs of precision,reproducibility and stability tests were ≤1.36%;recoveries were 97.11-99.87%(RSD=0.95%,n=9)and 98.47-101.99%(RSD=1.08%,n=9),respectively.CONCLUSIONS:
The method is simple,accurate and reliable,and can be used for contents determination of tetrahydropalmatine and rosmarinic acid in Xiaoheling capsule.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2015
Type:
Article
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