Your browser doesn't support javascript.
loading
Simultaneous Contents Determination of Tetrahydropalmatine and Rosmarinic Acid in Xiaoheling Capsule by HPLC / 中国药房
China Pharmacy ; (12): 4719-4721, 2015.
Article in Chinese | WPRIM | ID: wpr-500886
ABSTRACT

OBJECTIVE:

To establish a method for the contents determination of tetrahydropalmatine and rosmarinic acid in Xiaoheling capsule.

METHODS:

HPLC was performed on the column of Phenomenex C18 with mobile phase A of 0.1% phosphoric acid(adjusted pH to 6.0 with triethylamine)-methanol(4555,V/V) and (adjusted pH to 6.0 with triethylamine) B of 0.1% phos-phoric acid-methanol(5545,V/V)(gradient elution)at flow rate of 0.8 ml/min,detection wavelength was 282 nm(0-25 min)and 330 nm(25.01-60 min)and volume size was 10 μl.

RESULTS:

The linear range was 1.502-75.10 μg/ml for tetrahydropalmatine and 6.03-301.6 μg/ml for rosmarinic acid(r=0.999 8);RSDs of precision,reproducibility and stability tests were ≤1.36%;recoveries were 97.11-99.87%(RSD=0.95%,n=9)and 98.47-101.99%(RSD=1.08%,n=9),respectively.

CONCLUSIONS:

The method is simple,accurate and reliable,and can be used for contents determination of tetrahydropalmatine and rosmarinic acid in Xiaoheling capsule.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: China Pharmacy Year: 2015 Type: Article

Similar

MEDLINE

...
LILACS

LIS

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: China Pharmacy Year: 2015 Type: Article