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Study on the HPLC Fingerprint Spectrum of Mongolian Medicine Rhaponticum uniflorum / 中国药房
China Pharmacy ; (12): 4690-4692, 2015.
Article in Chinese | WPRIM | ID: wpr-500893
ABSTRACT

OBJECTIVE:

To establish HPLC fingerprint sectrum of Rhaponticum uniflorum.

METHODS:

HPLC was performed on the column of Hypersil-ODS with mobile phase of 0.3% phosphoric acid-acetonitrile(gradient elution) at flow rate of 1.0 ml/min,detection wavelength was 220 nm,column temperature was 30 ℃ and volume injection was 10 μl. The luteolin was refer-ence,17 batches of R. uniflorum from different production places was analyzed and similarity evaluation system for chromatograph-ic fingerprint of TCM (2004 A edition)was adopted for the similarity analysis.

RESULTS:

There were totally 11 common peaks with similarity degree≥0.900 of 17 batches. According to the verification,the fingerprint spectrum and reference fingerprint spec-trum of R. uniflorum had good consistency.

CONCLUSIONS:

The established method is specific and stable,and can provide refer-ence for the identification and quality control of R. uniflorum.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: China Pharmacy Year: 2015 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: China Pharmacy Year: 2015 Type: Article