Study on the HPLC Fingerprint Spectrum of Mongolian Medicine Rhaponticum uniflorum / 中国药房
China Pharmacy
;
(12): 4690-4692, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-500893
ABSTRACT
OBJECTIVE:
To establish HPLC fingerprint sectrum of Rhaponticum uniflorum.METHODS:
HPLC was performed on the column of Hypersil-ODS with mobile phase of 0.3% phosphoric acid-acetonitrile(gradient elution) at flow rate of 1.0 ml/min,detection wavelength was 220 nm,column temperature was 30 ℃ and volume injection was 10 μl. The luteolin was refer-ence,17 batches of R. uniflorum from different production places was analyzed and similarity evaluation system for chromatograph-ic fingerprint of TCM (2004 A edition)was adopted for the similarity analysis.RESULTS:
There were totally 11 common peaks with similarity degree≥0.900 of 17 batches. According to the verification,the fingerprint spectrum and reference fingerprint spec-trum of R. uniflorum had good consistency.CONCLUSIONS:
The established method is specific and stable,and can provide refer-ence for the identification and quality control of R. uniflorum.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2015
Type:
Article
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