Content Determination of Eudesmin in Zanthoxylum simulans by HPLC / 中国药房

ABSTRACT

OBJECTIVE:To establish a method for the content determination of eudesmin in Zanthoxylum simulans. METH-ODSHPLC was performed on the column of Kromasil C18 with mobile phase of acetonitrile-water(5644,V/V)at flow rate of 1.0 ml/L,detection wavelength was 221 nm,column temperature was 25 ℃. RESULTS:The linear of eudesmin was 0.208-2.08 μg (r=0.999 8);RSDs of precision,reproducibility and stability tests were lower than 2.0%;recovery was 96.18%-103.04%(RSD=2.58,n=6). CONCLUSIONS:The method is simple and good reproducibility,and can be used for the content determination of eu-desmin in Z. simulans.