Content Determination of Vitamin B1 and Vitamin B2 in Trivitamin and Calcium Gluconate,Calcium Hydro-gen Phosphate Chewable Tablets by HPLC / 中国药房
China Pharmacy
;
(12): 2549-2551, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-500897
ABSTRACT
OBJECTIVE:
To establish a method for the content determination of vitamin B1 and vitamin B2 in Trivitamin and calci-um gluconate,calcium hydrogen phosphate chewable tablets.METHODS:
HPLC was conducted. The column was Inertsil C18 with the mobile phase of 1%triethylamine contained 0.02 mol/L of sodium heptane solution(pH was adjusted to 5.5 by phosphate)-methanol (74∶26,V/V)at the flow rate of 1.0 ml/min,the detection wavelength was 260 nm,temperature was 30℃and the volume was 20μl.RESULTS:
There was a good linear relationship between the quality concentration of vitamin B1 and peak area in the range of 0.22-1.10μg/ml(r=0.999 8)and vitamin B2 was in the range of 0.22-1.09μg/ml(r=0.999 7). The RSDs of precision,reproducibility and stabil-ity tests were all less than 1%;the average recovery was respectively 99.66%(RSD=0.96%,n=9)and 100.31%(RSD=0.92%,n=9).CONCLUSIONS:
The method is simple,accurate,sensitive and reliable and suitable for the content determination of vitamin B1 and vitamin B2 in Trivitamin and calcium gluconate,calcium hydrogen phosphate chewable tablets.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2015
Type:
Article
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