Study on Extraction Technology of Polysaccharides from Stemona tuberosa by Alkaline Water Ultrasound Method / 中国药房
China Pharmacy
;
(12): 2695-2697, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-500932
ABSTRACT
OBJECTIVE:
To optimize extraction technology of polysaccharides from Stemona tuberosa so as to increase the ex-traction rate and efficiency.METHODS:
Anthrone-sulfuric acid method was used for detection. With water and alkaline water (NaOH solution)as the solvent respectively,the effects of decocting method,ultrasound method and microwave method on the ex-traction rate of crude polysaccharide were observed respectively. By carrying out single factor and orthogonal tests,with the extrac-tion rate of crude polysaccharide as the index,the effects of the amount of water,NaOH solution concentration,extraction tempera-ture and extraction time on the extraction of crude polysaccharide by alkaline water ultrasound method were observed to optimize the technology parameters,and verification test was conducted.RESULTS:
The alkaline water ultrasound method is superior to oth-er extraction methods,where the optimal technology parameters were as follows as 0.3 mol/L NaOH aqueous solution 30 times as much as the amount of crud drug,ultrasonic processing for 70 min at 50 ℃. The verification tests showed that the average extrac-tion rate of crude polysaccharide was 25.76%(RSD=3.51%,n=6).CONCLUSIONS:
The optimal alkaline water ultrasound method can better extract crude polysaccharide from S. tuberosa.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2015
Type:
Article
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