Comparative Study on HPLC Characteristic Spectrum of Erycibe obtusifolia from Different Habitats by HPLC / 中国药房
China Pharmacy
;
(12): 3417-3419, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-501032
ABSTRACT
OBJECTIVE:
To establish and compare the HPLC characteristic Spectrum Erycibe obtusifolia from different habi-tats.METHODS:
HPLC was performed on the column of Hypersil ODS C18 with the mobile phase of acetonitrile-0.1% phosphoric acid at flow rate of 1.0 ml/min,detection wavelength was 347 nm,the column temperature was 30℃and the sample size was 20μl. 12 different batches of samples were determined and the characteristic spectrums of those were established. TCM fingerprint evalu-ation software(2004 edition)was used to evaluate the similarity of 12 batches of samples.RESULTS:
10 characteristic peaks were identified in the characteristic spectrum of E. obtusifolia. Peak 3 was scopolamine glycosides,peak 4 was chlorogenic acid and peak 6 was scopoletin. The similarities of 10 batches of samples were proved to be higher than 0.90 and 2 batches were proved to be less than 0.90.CONCLUSIONS:
The method is simple,accurate and reproducible,and can provide scientific evidence for effectively controlling the internal quality standards.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Prognostic study
Language:
Chinese
Journal:
China Pharmacy
Year:
2015
Type:
Article
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