Screening and Identification of Endophytic Fungi from Schisandra chinensis with Antioxidant Activity / 中国药房
China Pharmacy
;
(12): 4384-4388, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-501110
ABSTRACT
OBJECTIVE:
To screen and identify endophytic fungi from Schisandra chinensis with antioxidant activity. METH-ODSThe tissue isolation skill was used to isolate endophytic fungi from roots,leaves,stems and fruits of S. chinensis. And anti-oxidant activity of endophytic fungi were screened by DPPH radical scavenging assay and hydroxyl radical scavenging assay. The to-tal DNA were extracted;the 18S rDNA ITS were amplified and sequenced with primer ITS1 and ITS4;the results of sequencing were analyzed comparatively based on homology to confirm the classification of active strains.RESULTS:
23 strains were isolated from S. chinensis. GSR-12,isolated from roots of S. chinensis,had strong antioxidant activities. The scavenging rate on DPPH and the hydroxyl radical were 87.96% and 82.31% respectively. GSR-12 strain was identified as Clonostachys rosea by analyzed com-paratively.CONCLUSIONS:
1 strain of C. rosea,isolated from roots of S. chinensis,has strong antioxidant activity.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
/
Screening study
Language:
Chinese
Journal:
China Pharmacy
Year:
2015
Type:
Article
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