Improvement of HPLC in Concentration Determination of Lamotrigine in Human Serum / 中国药房

ABSTRACT

OBJECTIVE:To establish a rapid method for the concentration determination of lamotrigine (LTG) in human se-rum. METHODS:HPLC was adopted after serum samples treated with methanol. The column was Hypersil BDS C18(200 mm×4.6 mm,5μm)with the mobile phase of methanol-KH2PO4(45∶55,V/V)at the flow rate of 1 ml/min,the detection wavelength was 250 nm,and the temperature was 35℃. RESULTS:The linear range of LTG was 0.5-30.0 μg/ml(r=0.999 9). The RSDs of intra-day and inter-day were all less than 5% and the recovery was 101.96%-102.74%. CONCLUSIONS:The method is simple,accurate and fast,and suitable for the monitoring of LTG serum concentration.