Simultaneous Determination of Andrographlide and Dehydroandrographolide in Ganmaoqing Tablet by HPLC / 中国药房
China Pharmacy
;
(12): 1699-1701, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-501236
ABSTRACT
OBJECTIVE:
To establish a method for simultaneous determination of andrographlide and dehydroandrographolide in Ganmaoqing tablet.METHODS:
HPLC was performed on the column of Thermo BDS C18 with mobile phase of mathanol-water at a flow rate of 1.0 ml/min,detection wavelength was 225 nm for andrographlide and 254 nm for dehydroandrographolide,col-umn temperature was 30 ℃,and injection volume was 10 μl.RESULTS:
The linear range was 2.25-45.00 μg/ml for andrographlide (r=0.9998) and 1.00-20.00 μg/ml for dehydroandrographolid(r=0.9990);RSDs of precision,stability and reproducibility tests were no more than 0.8%;average recoveries were 96.96%-99.80%(n=9) and 96.79%-100.65%(n=9).CONCLUSIONS:
The method is simple,accurate and reliable,and can be used for the quality control of andrographlide and dehydroandrographolide in Ganmaoqing tablet.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2016
Type:
Article
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