Content Determination of Paeoniflorin in Bailing Tablet by HPLC and Its Uncertainty Evaluation / 中国药房
China Pharmacy
;
(12): 5152-5154, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-501324
ABSTRACT
OBJECTIVE:
To establish a method to determinate the content of paeoniflorin in Bailing tablet,and to evaluate the uncertainty of results.METHODS:
HPLC was performed on the column of Platisil C18 with mobile phase of water- acetonitrile(8713,V/V) at a flow rate of 1 ml/min,the detection wavelength was 230 nm,the column temperature was 25 ℃ and the injection volume was 10 μl. Quantitative analysis was conducted for the processes and procedures and the uncertainty was evaluated.RESULTS:
The linear range of paeoniflorin was 0.100 1-2.502 8 μg;RSDs of precision,stability and reproducibility tests were lower than 2.0%,recovery rate was 98.7%-100.9%(RSD=0.86%,n=6). Reference substance amount,elution produce and the accuracy of method had great effect on the uncertainty.CONCLUSIONS:
The established method can be used for the content determination of paeoniflorin in Bailing tablet,and the uncertainty evaluation provides reliability for the method.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2015
Type:
Article
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