Content Determination of Chlorogenic Acid in Yinhuang Qinghuo Capsule by HPLC / 中国药房
China Pharmacy
;
(12): 5147-5148, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-501326
ABSTRACT
OBJECTIVE:
To establish a method to determine the content of chlorogenic acid in Yinhuang qinghuo capsule.METHODS:
HPLC was performed on the column of Acclaim120? C18 with mobile phase of acetonitrile-0.4% phosphoric acid(1288,V/V)at flow rate of 1 ml/min,the detection wavelength was 327 nm,column temperature was 25 ℃,and volume injection was 10 μl.RESULTS:
The linear range of chlorogenic acid was 56-224 μg;RSDs of precision,stability and reproduciblity tests were lower than 3.0%,recovery was 96.91%-104.76%(RSD=1.46%,n=9).CONCLUSIONS:
The method is simple and reproducible, and can be used for the quality control of Yinhuang qinghuo capsule.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2015
Type:
Article
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