Stuoly of the Methods of Content Determination of Rosmarinic Acid and Total Polysaccharide in Hyssopus off-cinalis / 中国药房
China Pharmacy
;
(12): 5133-5135, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-501330
ABSTRACT
OBJECTIVE:
To establish a method for content determination of rosmarinic acid and total polysaccharide in Hysso-pus. offcinalis.METHODS:
HPLC was conducted to determine the content of rosmarinic acid in H. offcinalis. The column was Shim-pack VP-ODS with mobile phase of acetonitrile-0.5% formic(1783,V/V) at flow rate of 1.0 ml/min,detection wavelength was 330 nm,column temperature was 30 ℃,and the injection volume was 10 μl. UV-visible spectrophotometry was conducted to determine the content of total polysaccharide,detection wavelength was 620 nm.RESULTS:
The linear range was 10.86-54.30 μg/ml (r=0.999 8)for rosmarinic acid and 1.996-15.97 μg/ml for total polysaccharide(r=0.999 6);RSDs of precision,stability and re-producibility tests were lower than 1.0% and 2.0%,respectively;recoveries were 98.20%-101.79%(RSD=1.68%,n=6) and 96.62%-101.68%(RSD=1.88%,n=6).CONCLUSIONS:
The method is simple and reproducible,and can be used for the con-tents determination of rosmarinic acid and total polysaccharide in H. offcinalis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2015
Type:
Article
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