Application of LC-MS/MS Method for the Determination of Carbamazepine in Human Plasma and Exter-nal Quality Assessment / 中国药房
China Pharmacy
;
(12): 1044-1047, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-501357
ABSTRACT
OBJECTIVE:
To establish and validate the method for the determination of carbamazepine (CBZ) in human plas-ma,and to apply the method for external quality assessment.METHODS:
After precipitated with acetonitrile,the plasma sample was determined by LC-MS/MS. Using loratadine as internal standard,the determination was performed on Kromasil C18 column with mobile phase consisted of water (containing 0.1% formic acid)-acetonitrile (gradient elution) at flow rate of 0.6 ml/min and column temperature of 40 ℃. The ion transitions under MRM mode by ESI+ ionization were performed at m/z 237.1→194.0 and m/z 383.1→267.0 for CBZ and internal standard,respectively.RESULTS:
The linear range of CBZ were 5-1 000 ng/ml (r>0.998). The limit of quantitation was 5 ng/ml. RSDs of inter-day and intra-day were 1.00%-6.42%;relative deviation were -6.93%-0.32%. The external quality assessment of 5 samples were 679.0,475.0,104.0,29.2 and 26.2 ng/ml,respectively. The pass rate of assess-ment result was 100%.CONCLUSIONS:
The method is sensitive,accurate and specific. The method is applicable for the plasma concentration determination and external quality assessment of CBZ.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2016
Type:
Article
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