Your browser doesn't support javascript.
loading
Simultaneous Determination of Puerarin,Berberine Hydrochloride and Baicalin in Erxieting Syrup by HPLC / 中国药房
China Pharmacy ; (12): 394-396, 2016.
Article in Chinese | WPRIM | ID: wpr-501420
Responsible library: WPRO
ABSTRACT

OBJECTIVE:

To establish a method for simultaneous determination of puerarin,berberine hydrochloride and ba-icalin in Erxieting syrup.

METHODS:

HPLC was performed on the column of Kromasil-C18 with mobile phase of methanol-0.2%Phosphoric acid solution (gradient elution) at flow rate of 1.0 ml/min,column temperature was 30 ℃,detection wavelength was 275 nm,and the volume injection was 10 μl.

RESULTS:

The linear was 10.43-83.44 μg/ml for puerarin,10.71-85.68 μg/ml for ber-berine hydrochloride and 12.58-100.64 μg/ml for baicalin(r≥0.999 3);RSDs of precision,stability and reproduciblity tests were lower than 2%;recoveries were 98.25%-100.87%(RSD=0.92%,n=6),99.41%-101.40%(RSD=0.68%,n=6)and 98.13%-99.76%(RSD=0.57%,n=6),respectively.

CONCLUSIONS:

The method is simple,reliable,can be used for the quality control of Erxiet-ing syrup.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: China Pharmacy Year: 2016 Type: Article

Similar

MEDLINE

...
LILACS

LIS

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: China Pharmacy Year: 2016 Type: Article