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Content Determination of Carbohydrates in Osseous and Cucumis Extract Injection by Ion Chromatography / 中国药房
China Pharmacy ; (12): 386-389, 2016.
Article in Chinese | WPRIM | ID: wpr-501422
ABSTRACT

OBJECTIVE:

To establish a method for the content determination of carbohydrates in Osseous and cucumis extract injection.

METHODS:

Ion chromatography was conducted on the column of Dionex CarboPac PA20 with mobile phase of water-1 mol/L Sodium acetate solution-250 mmol/L Sodium hydroxide solution(gradient elution)at flow rate of 0.45 ml/min,column tem-perature was 35 ℃,and injection volume was 10 μl,detector was electrochemical analysis detector.

RESULTS:

The linear ranges of galactose,glucose,mannose,sucrose and fructose were 0.5-10.0μg/ml(r>0.998 0);RSDs of precision,stability and reproduc-ibility tests were lower than 2%;recovery was 94.8%-99.1%(RSD=0.52%-0.89%,n=9).

CONCLUSIONS:

The method is sim-ple,fast and accurate,and can be used for the determination of carbohydrates in Osseous and cucumis extract injection.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: China Pharmacy Year: 2016 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: China Pharmacy Year: 2016 Type: Article