Content Determination of Carbohydrates in Osseous and Cucumis Extract Injection by Ion Chromatography / 中国药房
China Pharmacy
;
(12): 386-389, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-501422
ABSTRACT
OBJECTIVE:
To establish a method for the content determination of carbohydrates in Osseous and cucumis extract injection.METHODS:
Ion chromatography was conducted on the column of Dionex CarboPac PA20 with mobile phase of water-1 mol/L Sodium acetate solution-250 mmol/L Sodium hydroxide solution(gradient elution)at flow rate of 0.45 ml/min,column tem-perature was 35 ℃,and injection volume was 10 μl,detector was electrochemical analysis detector.RESULTS:
The linear ranges of galactose,glucose,mannose,sucrose and fructose were 0.5-10.0μg/ml(r>0.998 0);RSDs of precision,stability and reproduc-ibility tests were lower than 2%;recovery was 94.8%-99.1%(RSD=0.52%-0.89%,n=9).CONCLUSIONS:
The method is sim-ple,fast and accurate,and can be used for the determination of carbohydrates in Osseous and cucumis extract injection.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2016
Type:
Article
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