Simultaneous Determination of Triamcinolone Acetonide Acetate and Tretinoin in Dimensional Ointment by HPLC / 中国药房
China Pharmacy
;
(12): 381-383, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-501424
ABSTRACT
OBJECTIVE:
To establish a method for the contents determination of triamcinolone acetonide acetate and tretinoin in Dimensional ointment.METHODS:
HPLC was performed on the column of Diamonsil C18 with mobile phase of methanol-0.1%Phosphoric acid solution(8713,V/V),detection wavelength was 254 nm(0-5 min)and 350 nm(5-20 min),at a flow rate of 1.0 ml/min,column temperature was 25℃,and volume injection was 10 μl.RESULTS:
The linear range was 0.5-5 μg/ml for triamcin-olone acetonide acetate(r=0.999 9)and 4.96-49.6 μg/ml for tretinoin(r=0.999 8);RSDs of precision,stability and reproducibility tests were lower than 1%;recoveries were 98.37%-100.03%(RSD=0.58%,n=6)and 98.21%-100.38%(RSD=0.78%,n=6). CON-CLUSIONSThe method is simple,accurate and fast,and suitable for the contents determination of triamcinolone acetonide ace-tate and tretinoin in Dimensional ointment.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2016
Type:
Article
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