Simultaneous Determination of Syringin and Pedunculoside in Zhuang Medicine Yuyang Powder by HPLC / 中国药房
China Pharmacy
;
(12): 818-820, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-501460
ABSTRACT
OBJECTIVE:
To establish a method for the simultaneous determination of syringinand and pedunculoside in Zhuang medicine Yuyang powder.METHODS:
HPLC was performed on the column of Agilent ODS with mobile phase of acetonitrile-water (gradient elution)at a flow rate of 1 ml/min,the detection wavelength was 210 nm,the column temperature was 30℃,and the in-jection volume was 5μl.RESULTS:
The linear range was 0.71-3.55μg for syringin(r=0.999 7)and 1.62-8.10μg for pedunculoside (r=0.999 8), respectively;RSDs of precision, stability and reproducibility tests were lower than 3%;recoveries were 100.8%-104.9%(RSD=1.7%,n=6) and 96.0%-100.8%(RSD=2.2%,n=6).CONCLUSIONS:
The method is simple,stable and reproducible,and can be used for the simultaneous determination of syringinand and pedunculoside in Zhuang medicine Yuyang powder.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2016
Type:
Article
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