Study on Contents Determination of Phospholipid andγ-linolenic Acid in Compound Linolenic Acid Soft Cap-sule / 中国药房
China Pharmacy
;
(12): 412-414, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-501475
ABSTRACT
OBJECTIVE:
To establish a method for the contents determination of phospholipid and γ-linolenic acid in Com-pound linolenic acid soft capsule.METHODS:
HPLC was conducted to determine the content of soybean lecithin;the column was Agilent TC-C18 with mobile phase of methanol-water(8416,V/V)at a flow rate of 1.0 ml/min,the detection wavelength was 219 nm,the column temperature was 30 ℃,and the injection volume was 10 μl.Gas chromatography was conducted to determine the content of γ-linolenic acid in the preparation;the column was DB WAX,injector temperature was 210 ℃ by temperature pro-grammed,carrier gas was helium at a flow rate of 1 ml/min by split injection(split ratio of 130),and the injection volume was 0.02μl.RESULTS:
The linear range was 4.64-16.24μg/ml for phospholipid(r=0.999 6)and 0.093 44-0.327 04μg/ml forγ-linole-nic acid methy ester(r=0.999 6);RSDs of precision,stability and reproducibility tests were lower than 3%;recoveries were 97.44%-99.36%(RSD=0.93%,n=6) and 97.22%-99.07%(RSD=1.01%,n=6),respectively.CONCLUSIONS:
The method is simple,stable with good separation,and can be use for the contents determination of phospholipid and γ-linolenic acid in Com-pound linolenic acid soft capsule.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2016
Type:
Article
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