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Inhibitory effect of paeoniflorin on inflammatory chemokines and their receptor in a mouse model of asthma / 中国实验动物学报
Article in Zh | WPRIM | ID: wpr-501593
Responsible library: WPRO
ABSTRACT
Objective To study the effect of paeoniflorin on inflammatory chemokines and their receptor in a rat model of ovalbumin-induced asthma.Methods Sixty 6-week old SPF female BALB/c mice were used in this study.To es-tablish a mouse model of asthma by sensitizing and challenging with ovalbumin.ELISA was used to analyze the serum IL-6 and TNF-α, and the specific IgE against ovalbumin ( OVA-IgE ) , CCL19 and CCL21 in bronchoalveolar lavage fluid (BALF).RT-PCR was performed to determine CCR7mRNA and protein expression of chemokine receptor CCR7, and the level of NF-κB was tested by Western blot.ResultsIn In the paeoniflorin groups, the serum IL-6 and TNF-αlevels were significantly lower, and the OVA-IgE, CCL19 and CCL21 levels in BALF were significantly reduced, compared with that in the control group.CCR7mRNA and protein expression of chemokine receptor CCR7 and NF-κB in the lung were significant-ly reduced by paeoniflorin.Conclusions Paeoniflorin has a remarkably inhibitory effect on the airway inflammatory chemo-kines CCL19/CCL21 and the receptor CCR7 in the mouse model of asthma.
Key words
Full text: 1 Index: WPRIM Language: Zh Journal: Acta Laboratorium Animalis Scientia Sinica Year: 2016 Type: Article
Full text: 1 Index: WPRIM Language: Zh Journal: Acta Laboratorium Animalis Scientia Sinica Year: 2016 Type: Article