The study on microRNA-451 expression and its association with gastric cancer / 中华普通外科杂志

ABSTRACT

Objective To investigate the relations between microRNA-451 (miR-451) expression and gastric cancer (GC).Methods Expression of miR-451 in tumor and paired non-cancerous tissues was measured by real-time quantitative polymerase chain reaction.The association of miR-451 expression with clinical pathological characteristics and prognosis was statistically analyzed;in addition,slow virus LV-mir-451 was applied to infect human gastric cancer cell line BGC-823 and MKN-45,and construct miR-451 over-expressed stable cell lines.Finally,to detect cell proliferation,migration and invasion.Results miR-451 expression decreased in GC tissues vs.pericarcinous tissues (0.010 ± 0.007 vs.0.014 ± 0.008,t =3.015,P ＜ 0.05),and its down-regulation positively correlated with lymphatic metastasis,clinical staging and shorter overall survival.miR-451 expression in BGC-823 and MKN-45 cells did not impact on cell proliferation,but did reduce migration rates (0.863 ±0.026 vs.1.107 ±0.032,t =9.820,P＜0.05;0.863 ±0.026 vs.1.060±0.054,t =4.369,P＜0.05) and invasion rates (1.040 ± 0.055 vs.1.522 ± 0.001,t =5.84,P ＜ 0.05;1.040 ± 0.055 vs.1.696 ± 0.078,t =10.16,P ＜ 0.05) in BGC-823 cells.Conclusions miR-451 may act as a prognostic marker in GC.