Role of JNK signaling pathway in hyperoxia-induced expression of connective tissue growth factor in A549 cells / 中华麻醉学杂志

ABSTRACT

Objective To investigate the role of c-Jun N-terminal kinase (JNK) signaling pathway in hyperoxia-induced expression of connective tissue growth factor (CTGF) in A549 cells.Methods The cultured A549 cells were seeded in 6-well culture plates at a density of 1 × 105 cells/ml (0.5 ml/well,24 wells in total) and were randomly divided into 4 groups (n =6 each) using a random number tablecontrol group (group C);95％ oxygen group (group 95％ O2);room air plus SP600125 (JNK inhibitor) group (group C+SP);95％ oxygen plus SP600125 group (group 95％ O2+SP).SP600125 5 μmol/L was added to each well in C+SP and 95％ O2+SP groups.The A549 cells were incubated for 48 h in an incubator filled with room air (C and C+SP groups) or 95％ oxygen (95％ O2 and 95％ O2+SP groups).The expression of phosphorylated JNK (p-JNK) and CTGF mRNA was determined using Western blot and realtime reverse transcriptase polymerase chain reaction,respectively.Results Compared with group C,no significant change was found in the expression of p-JNK and CTGF mRNA in group C+SP (P＞0.05),and the expression of p-JNK and CTGF mRNA was significantly up-regulated in group 95％ O2 (P＜ 0.05).Compared with group 95％ O2,the expression of p-JNK and CTGF mRNA was significantly down-regulated in group 95％ O2+SP (P＜0.05).Conclusion JNK signaling pathway activation is involved in up-regulation of hyperoxia-induced CTGF mRNA expression in A549 cells.