Cardiac stem cells:isolation, culture, proliferation and migration / 中国组织工程研究
Chinese Journal of Tissue Engineering Research
;
(53): 6190-6196, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-503402
ABSTRACT
BACKGROUND:
In the process of cardiac stem cel culture in vitro, the growth microenvironment may have some effects on the cel proliferation.OBJECTIVE:
To investigate the possible mechanism of proliferation and migration of rat cardiac stem cel s cultured in vitro.METHODS:
Cardiac tissues from 10 Sprague-Dawley rats were obtained for primary culture and subculture. Passage 3 cel s were col ected for immunofluorescence staining, and stem cel growth factor receptor (c-kit) and CD45, CD90 were detected. Cultured tissues were col ected and randomly divided into two groupsin group 1, paraformaldehyde fixation, paraffin embedding, hematoxylin-eosin staining, Masson staining, and detecting apoptotic cel s using TUNEL method were conducted;in group 2, EdU labeling of proliferated cel s, immunofluorescent detection of c-kit positive expression, matrix metal oproteinases 2, 9 and transforming growth factorβ1 using immunofluorescent staining were done. RESULTS ANDCONCLUSION:
After 7-10 days of myocardial tissue culture, bright and round cel s were visible, and after adhesion, fusiform cel s exhibited strong growth and proliferation ability. Immunofluorescence staining showed a large number of c-kit, CD45 positive cel s but CD90 negative cel s. After culture, a great number of newborn cel s were found, accompanied by apoptosis of myocardial cel s. After EdU staining, the positive cel s were distributed in the myocardial gap, and showed a smal amount of matrix metal oproteinases 2, 9 and transforming growth factorβ1, while in the surrounding myocardium there was a large number of matrix metal oproteinases 2, 9 and transforming growth factorβ1. Taken together, our findings show that cardiac stem cel s could be obtained through myocardial tissue culture in vitro, accompanied by cel proliferation and migration. The mechanism is related to the expression of matrix metal oproteinases 2, 9 and transforming growth factorβ1.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Tissue Engineering Research
Year:
2016
Type:
Article
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