Prokaryotic expression and purification of SET7 with GST-tag / 军事医学
Military Medical Sciences
;
(12): 707-709, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-503987
ABSTRACT
Objective To purify and prokaryotically express the histone methyltransferase SET7.Methods The coding sequence of full length SET7 was amplified from breast library by PCR and cloned into the pGEX-KG vector.The correct recombinant plasmid was introduced into E.coli.The expressed protein was identified by SDS-PAGE.Results DNA sequencing indicated the SET7 was constructed.GST-SET7 fusion protein was identified by SDS-PAGE.Conclusion The prokaryotically expressed protein of GST-SET7 is obtained,which will falilitate further study of SET7 function.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Military Medical Sciences
Year:
2016
Type:
Article
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