Effect of astragaloside IV on neurological function in acute cerebral he-morrhage rats / 中国病理生理杂志

ABSTRACT

[ ABSTRACT] AIM:To investigate the effect of astragaloside IV on neurological function and the protein expres -sion of neuron-specific enolase (NSE), Notch1 and NF-κB in acute cerebral hemorrhage (ACH) rats.METHODS:ACH model in rats was established via injection of autologous blood , and the rats were divided into 4 groupssham, ACH, ACH+astragaloside IV (100 mg/kg) and ACH+astragaloside IV (200 mg/kg) groups.The impairment of neurological func-tion in each group was graded , and the brain coefficient and water content were calculated .The serum level of NSE was measured by ELISA.Additionally, the expression of Notch1 and NF-κB was determined by Western blot .RESULTS:As-tragaloside IV improved the neurological function and decreased the brain coefficient and water content in ACH rats .Moreo-ver, the ACH-induced increase in NSE was inhibited after astragaloside IV treatment .Similarly, astragaloside IV also sig-nificantly attenuated the expression of Notch 1 and NF-κB in ACH rats.CONCLUSION: Astragaloside IV attenuates the impairment of neurological function in ACH rats , which may be through decreasing the NSE level and down-regulating the expression of Notch1 and NF-κB.