Content Determination of Garlic Saponin in Allium sativum by Pre-column Derivatization-HPLC / 中国药房
China Pharmacy
;
(12): 361-364, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-504317
ABSTRACT
OBJECTIVE:
To establish a method for content determination of steroidal saponin in Allium sativum.METHODS:
Pre-column derivatization of steroidal saponin was performed by using the derivatization agent of nitro-benzoic acid-chlorine. And HPLC was conducted to determine the content of steroidal saponin. The column was Shimadzu VP-ODS with mobile phase of aceto-nitrile-water mixed solution(8020,V/V)at a flow rate of 1.0 ml/min,the detection wavelength was 254 nm,the column temper-ature was 25 ℃,and the injection volume of 20 μl.RESULTS:
The linear rang of sarsasapogenin was 0-1.25 mg/ml(r=0.999 0);RSDs of precision,stability and reproducibility tests were lower than 2%;recovery was 93.1%-96.8%(RSD=1.56%,n=6). CON-CLUSIONSThe method is simple,stable with good separation,and can be use for the content determination of steroidal saponin in A. sativum.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2016
Type:
Article
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