Study on the Effect of Vitamin E Against Methylmercury-induced Neurotoxicity in Cultured Spinal Motor Neurons / 체질인류학회지
Korean Journal of Physical Anthropology
;
: 109-116, 1996.
Article
in Korean
| WPRIM
| ID: wpr-50433
ABSTRACT
In order to investigate the neurotoxic effect of methylmercury (MM) on cultured mouse spinal motoneuron cell line, NSC-19, neurotoxic effect of MM was evaluated by MTT assay after neurons were incubated with various concentrations of methylmercuric chloride (MMC) for 24 hours. In addition, neuroprotective effect of vitamin E against MMC-induced neurotoxicity was examined by MTT assay and neurofilament enzymeimmunoassay (EIA) in these cultures. The results were as follows 1. MTT50 value was a concentration of 20µM methylmercuric chloride. 2. Methylmercuric chloride was toxic on cultured spinal motor neurons, NSC-19 cells in a time-and dose-dependent manner by severe decrease of cell viability. 3. Methylmercuric chloride induced the decrease of cell number and the loss of neuritis on these cultures. 4. Vitamin E remarkably increased the viabilily of cultured neurons damaged by methylmercury-induced neurotoxicity at a concentration of 250µM vitamin E. From above the results, it is suggested that methylmercury induces severe toxic effect on cultured mouse spinal motor neurons, NSC-19 cells, and the selective antioxidants such as vitamin E are effective in the neurotoxicity induced by methylmercury in these cultures.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Vitamin E
/
Vitamins
/
Intermediate Filaments
/
Cell Count
/
Cell Line
/
Cell Survival
/
Neuroprotective Agents
/
Cell Culture Techniques
/
Motor Neurons
/
Neuritis
Limits:
Animals
Language:
Korean
Journal:
Korean Journal of Physical Anthropology
Year:
1996
Type:
Article
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