Distribution of Levofloxacin in Lung Tissue of Mice after Intragastric and Intravenous Administration of Tanreqing Injection Combined with Levofloxacin / 中国药房

ABSTRACT

OBJECTIVE:To compare the distribution of levofloxacin in lung of mice after intragastric and intravenous adminis-tration of Tanreqing injection combined with levofloxacin. METHODS:144 mice were randomly divided into experimental group and control group,with 72 mice in each group. Both groups were given Tanreqing injection(0.8 ml/kg). 1 h later,control group was given Levofloxacin injection(80 mg/kg)intravenously,and experimental group Levofloxacin tablet solution(80 mg/kg)intra-gastrically;every 8 mice were sacrificed for lung tissue collection at the time points of 0.05,0.083,0.17,0.5,1.0,2.0,4.0,8.0, 12.0 h and 0.083,0.17,0.33,0.5,1.0,2.0,4.0,8.0,12.0 h,respectively. Using ciprofloxacin as internal standard,the content of levofloxacin in lung tissue was determined by HPLC,and pharmacokinetic parameters were calculated by 3p97 program. RE-SULTSThe concentration-time curves of levofloxacin in lung tissue were in line with two-compartment model in 2 groups. The main pharmacokinetics of experimental group and control group were as follows as t1/2β of(4.17±0.84)h and(4.10±0.55)h;CL of(0.66±0.049)L/h and(0.71±0.21)L/h;AUC0-t of(109.48±12.34)mg·h/kg and(113.04±29.43)mg·h/kg;cmax of(38.76± 1.62) mg/kg and (42.28 ± 2.03) mg/kg,respectively. There was no statistical significance (P>0.05). Absolute bioavailability of levofloxacin was(96.85±17.39)% in experimental group with intragastric administration. CONCLUSIONS:After intravenous injec-tion of Tanreqing injection,the distribution of levofloxacin in lung tissue of mice are similiar between intragastric administration and intragastric administration.