Over-expression vector construction of human DcR3 gene and its validation / 中国免疫学杂志
Chinese Journal of Immunology
;
(12): 1491-1495, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-504371
ABSTRACT
Objective:
To construct the human DcR3 expression vector and verify its expression in vitro.Methods:
915 bp human DcR3 gene CDS was amplified from porcine lung tissues,and was cloned into eukaryotic expression vector pEF1a-IRES-DsRed-Express2 which show red fluorescence. And then pEF1a-IRES-DsRed-Express2-DcR3 was transfected into LX-2 cells by FuGene HD. Expression of mRNA and protein lever of Human DcR3 were detected by RT-PCR and Western blot.Results:
The levels of DcR3 gene transcription and translation in the hepatic stellate cells were significantly increased after transfection with pEF1a-IRES-DsRed-Ex-press2-DcR3 by RT-PCR and Western blot analysis.Conclusion:
DcR3 expression vector was successfully constructed and highly expressed in LX-2 cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Immunology
Year:
2016
Type:
Article
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