Induction Effect and Mechanism of Entecavir on the Apoptosis of Hepatocellular Carcinoma HepG2 Cells / 中国药房

ABSTRACT

OBJECTIVE:To study induction effect of entecavir on the apoptosis of hepatocellular carcinoma HepG2 cells and its mechanism. METHODS:After treated with 0(normal control),10,30 and 100 μmol/L(low,medium and high concentration groups)entecavir for 48 h,MTT method was adopted to detect HepG2 cell viability. AnnexinⅤ-PI flow double staining was used to detect cell apoptosis. Western blot was used to determine the phosphorylation of nuclear factor kappa B p65(NF-κB p65)and nu-clear factor kappa B inhibitor α(IκBα),and the protein expression of Bax,Bcl-2,Survivin and C-myc. RESULTS:Compared with normal control group,the cell viability,the phosphorylation of NF-κB p65 and IκBα,and the protein expression of Survivin, C-myc and Bcl-2 of entecavir low,medium and high concentration groups all decreased;the apoptotic rate,the protein expression of Bax increased(P<0.05 or P<0.01),in concentration-dependent manner. CONCLUSIONS:Entecavir can decrease viability of HepG2 cells and induce cell apoptosis,which is related to up-regulation expression of Bax,down-regulation expression of Sur-vivin,C-myc and Bcl-2,and blocking the activation of NF-κB/IκBαsignaling pathway.