Separation and Preparation of 5 Phenylpropanoids from Cinnamomum Cassia by Semi-preparative HPLC and Their Immunosuppressive Activity Investigation / 中国药师
China Pharmacist
;
(12): 1625-1628, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-504592
ABSTRACT
Objective:
To simultaneously separate 5 phenylpropanoids from Cinnamomum cassia by semi-preparative HPLC, and explore their immunosuppressive activity.Methods:
After extracted by ethanol, the ethyl acetate part of Cinnamomum cassia was isola-ted by semi-preparative HPLC. The separation was conducted on an Ultimate XB-C18 (250 mm × 10 mm, 5μm) semi-preparative chro-matographic column and the mobile phase was acetonitrile-water with gradient elution. The detection wavelength was 330 nm, and the flow rate was 2. 5 ml·min-1 . The sample volume was 0. 3 ml. MS and NMR spectroscopic analysis were used to determine the config-urations. A CCK-8 method was used to detect the immunosuppressive activity of phenylpropanoids.Results:
Totally 5 phenylpro-panoids were separated from Cinnamomum cassia by the semi-preparative HPLC, and identified as erythro-guaiacylglycerol, (7R,8S)-syringoylglycerol, (7S,8S)-syringoylglycerol, 3-methoxyphenyl-acrylaldehyde and O-methoxy cinnamaldehyde. The inhibitory rates of T cells and B cells of the compound 3 was more than 20% at the concentration of 800μmol·L-1 .Conclusion:
The method is conven-ient with good separation effect, which can simultaneously separate 5 phenylpropanoids from Cinnamomum cassia, and among them, the compound 3 shows immunosuppressive effect to some extent.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacist
Year:
2016
Type:
Article
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