Simultaneous Determination of Three Active Compounds in Heye Granule by RP-HPLC / 中国药房
China Pharmacy
;
(12): 2532-2534, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-504657
ABSTRACT
OBJECTIVE:
To establish a method for simultaneous determination of rutin,quercetin and nuciferine in Heye gran-ule.METHODS:
HPLC was performed on the column of Hypersil ODS2 with mobile phase of acetonitrile-water(containing 0.3%phosphoric acid and 0.4% triethylamine)(gradient elution)at a flow rate of 1.0 ml/min,the detection wavelength was 256 nm,the column temperature was 25 ℃,and the injection volume was 10 μl.RESULTS:
The linear range was 0.012-0.240 μg for rutin(r=0.999 9),0.010 4-0.208 μg for quercetin(r=0.999 9)and 0.015-0.300 μg for nuciferine(r=0.999 9);RSDs of precision,stability and reproducibility tests were lower than 2.0%;recoveries were 98.5%-101.3%(RSD=1.1%,n=6),99.1%-101.6%(RSD=1.0%, n=6)and 98.9%-101.7%(RSD=1.2%,n=6).CONCLUSIONS:
The method is simple,accurate and reliable,and can provide ref-erence for quality control of Heye granule.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2016
Type:
Article
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