Simultaneous Determination of Lithospermic Acid and Rosmarinic Acid in Guanxin Danshen Capsule by UPLC / 中国药房
China Pharmacy
;
(12): 2560-2562, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-504668
ABSTRACT
OBJECTIVE:
To establish a method for the simultaneous determination of lithospermic acid and rosmarinic acid in Guanxin danshen capsule.METHODS:
UPLC was performed on the column of ACQUITY UPLC-BEH C18 with mobile phase of 0.1% formic acid-methanol solution (gradient elution) at a flow rate of 0.3 ml/min,detection wavelength was 280 nm,column temperature was 30 ℃,and the injection volume was 5 μl.RESULTS:
The linear range was 0.019-0.76 mg/ml for lithospermic ac-id(r=0.999 9)and 0.005-0.2 mg/ml for rosmarinic acid(r=0.999 7);RSDs of precision,stability and reproducibility tests were lower than 2%;recoveries were 97.34%-101.24%(RSD=1.57%,n=6) and 98.30%-100.39%(RSD=0.86%,n=6). CONCLU-SIONSThe method is simple,accurate and reproducible,and can be used for the contents determination of lithospermic acid and rosmarinic acid in Guanxin danshen capsule.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2016
Type:
Article
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