Content Determination of Kaempferol in Kaempferia galanga by HPLC / 中国药房
China Pharmacy
;
(12): 2558-2559, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-504669
ABSTRACT
OBJECTIVE:
To establish a method for the content determination of kaempferol in Kaempferia galanga. METH-ODSHPLC was performed on the column of Diamonsil ODS2 C18 with mobile phase of methanol-0.4% Phosphoric acid solution at a flow rate of 1 ml/min,detection wavelength was 367 nm,column temperature was 30℃,and injection volume was 10 μl. RE-SULTSThe linear range of kaempferol was 0.001 58-0.158 mg/ml;RSDs of precision,stability and reproducibility tests were low-er than 3%;recovery was 95.52%-99.32%(RSD=1.47%,n=6).CONCLUSIONS:
The method is simple,accurate and reproduc-ible,and can be used for the content determination of kaempferol in K. galanga.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2016
Type:
Article
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