Regulatory Effect of Lentinan on Immune Function of Ulcerative Colitis Rats and Its Mechanism Study / 中国药房

ABSTRACT

OBJECTIVE:To study the regulatory effect of lentinan (LTN) on immune function in ulcerative colitis (UC) rats and its mechanism. METHODS:64 SD rats were randomly divided into normal group(12 rats)and modeling group(52 rats). UC model was induced in modeling group by a compound method of 2,4-dinitrochlorobenzene(DNCB)acetone and 2,4-DNCB etha-nol. After modeling, rats were randomly divided into model group, positive group (sulfasalazine tablet, 300 mg/kg), LTN low-dose,medium-dose and high-dose groups(0.2,0.4,0.8 mg/kg),with 10 rats in each group. Treatment groups were given rele-vant medicine intragastrically,and normal group and model group were given normal saline intragastrically,once a day,for consec-utive 15 d. The levels of CD3+,CD4+,CD8+in peripheral blood lymphocytes(PBMC)and the levels of TNF-α,IL-2,IL-4 and IL-10 in colon were all determined,and the pathological changes of colon tissue were observed. RESULTS:Compared with normal group,the levels of CD3+,CD4+,CD8+,the ratio of CD4+/CD8+,and the levels of IL-4 and IL-10 decreased significantly,while the levels of TNF-α and IL-2 in colon tissue significantly increased(P<0.05);obvious ulcerative lesion was observed in colon tissue (concentrated in grade Ⅱ and Ⅲ). Compared with model group,the levels of CD3+,CD4+,CD8+,the ratio of CD4+/CD8+,and the levels of IL-4,IL-10 increased significantly,while the levels of TNF-α,IL-2 in colon tissue significantly decreased (P<0.05);the inflammation of colon tissue was significantly reduced,and the ulcer formation was significantly improved. CONCLUSIONS:LTN has a certain improvement effect on UC model rats,and its mechanism may be related to enhancing the immune function.