Study on the Quality Standard of Flemingia philippinensis / 中国药房
China Pharmacy
;
(12): 3400-3403, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-504934
ABSTRACT
OBJECTIVE:
To establish the quality standard of Flemingia philippinensis.METHODS:
The properties and micro-scopic characteristics were observed;TLC was adopted for qualitative identification of genistin and genistein;the moisture,total ash and extract were detected;HPLC was adopted for contents determination of genistin and genisteinthe column was Thermo BE-TASIL C18 with mobile phase of Acetonitrile-0.5% glacial acetic acid(gradient elution)at a flow rate of 1.0 ml/min,the detection wavelength was 261 nm,column temperature was 25℃,and the injection volume was 10μl.RESULTS:
The properties and micro-scopic characteristics of F. philippinensis and F. macrophylla showed strong specificity. TLC spots of genistin and genistein we- re clear and well separated,with no interference in negative control. The moisture was 3.69%-8.37%,total ash was 1.72%-6.74%,and extract was 5.89%-19.65%. The linear range was 0.012 9-2.588 μg for genistin(r=0.999 9)and 0.004 6-0.923 2 μg for genistein (r=0.999 9);RSDs of precision,stability and reproducibility tests were lower than 3.0%;recoveries were 99.63%-101.87%(RSD=0.82%,n=6)and 97.19%-100.34%(RSD=1.23%,n=6).CONCLUSIONS:
The method is simple,accurate and specif-ic,and can be used for the quality control of F. philippinensis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Prognostic study
/
Qualitative research
Language:
Chinese
Journal:
China Pharmacy
Year:
2016
Type:
Article
Similar
MEDLINE
...
LILACS
LIS