Effect of cell-penetrating peptide-conjugated estrogen-related receptor beta on the development of mouse embryos cultured in vitro / 대한생식의학회지
Clinical and Experimental Reproductive Medicine
;
: 1-8, 2014.
Article
in English
| WPRIM
| ID: wpr-50505
ABSTRACT
OBJECTIVE:
Estrogen related receptor beta (Esrrb) is a member of the orphan nuclear receptors and may regulate the expression of pluripotency-related genes, such as Oct4 and Nanog. Therefore, in the present study, we have developed a method for delivering exogenous ESRRB recombinant protein into embryos by using cell-penetrating peptide (CPP) conjugation and have analyzed their effect on embryonic development.METHODS:
Mouse oocytes and embryos were obtained from superovulated mice. The expression of Oct4 mRNA and the cell number of inner cell mass (ICM) in the in vitro-derived and in vivo-derived blastocysts were first analyzed by real time-reverse transcription-polymerase chain reaction and differential staining. Then 8-cell embryos were cultured in KSOM media with or without 2 microg/mL CPP-ESRRB protein for 24 to 48 hours, followed by checking their integration into embryos during in vitro culture by Western blot and immunocytochemistry.RESULTS:
Expression of Oct4 and the cell number of ICM were lower in the in vitro-derived blastocysts than in the in vivo-derived ones (p<0.05). In the blastocysts derived from the CPP-ESRRB-treated group, expression of Oct4 was greater than in the non-treated groups (p<0.05). Although no difference in embryonic development was observed between the treated and non-treated groups, the cell number of ICM was greater in the CPP-ESRRB-treated group.CONCLUSION:
Treatment of CPP-ESRRB during cultivation could increase embryos' expression of Oct4 and the formation rate of the ICM in the blastocyst. Additionally, an exogenous delivery system of CPP-conjugated protein would be a useful tool for improving embryo culture systems.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oocytes
/
Blastocyst
/
RNA, Messenger
/
Immunohistochemistry
/
Cell Count
/
Blotting, Western
/
Embryonic Development
/
Embryonic Structures
/
Estrogens
/
Orphan Nuclear Receptors
Limits:
Animals
/
Pregnancy
Language:
English
Journal:
Clinical and Experimental Reproductive Medicine
Year:
2014
Type:
Article
Similar
MEDLINE
...
LILACS
LIS