Feasibility study on the application of the heterogeneous de protein cancellous bone in the treatment of lumber intertransverse process spinal fusion / 中国医师杂志

ABSTRACT

Objective To investigate application feasibility of the heterogeneous de protein cancellous bone in the treatment of lumber intertransverse process spinal fusion.Methods Thirty 6 ～ 8 month healthy male goats were selected to make intertransverse bone bed,and were randomly divided into groups Ⅰ and Ⅱ,with 15 in each group.Group Ⅰ was the left side implanted the tissue engineering bone (A group),and the right side implanted recombinant human bone morphogenetic protein-2 (rhBMP-2) composite bone (B group);Group Ⅱ was the left implanted autologous bone (C group),and the right side implanted simple heterogeneous de protein cancellous bone (D group).Each group was observed with the Xray,histological morphology,and maximum bending load.Results (1) In groups B and D,postoperative 4-,8-and 12-week X-ray scores were lower than that of group C (P ＜0.05);In group A,postoperative 4-,8-and 12-week X-ray score were (5.30 ± 0.41) points,(8.54 ± 0.89) points,and (11.25 ± 0.91)points,compared to group C,without statistically significant difference (P ＞ 0.05);(2) For groups A,B and D,postoperative 4-and 8-week histological scores were significantly lower than those in group C (P ＜0.05).For group A,postoperative 4-and 8-week histological scores were significantly higher than those of groups B and D (P ＜0.05).The histological scores of groups B and D postoperative 12 weeks were lower than those of group C (P ＜0.05).The histological score of group A postoperative 12 weeks was (11.04 ± 1.52) points,compared to group C without statistically significant difference (P ＞ 0.05);(3) The maximum bending load of groups B and D was lower than that of group C (P ＜ 0.05).The maximum bending load of group A was (60.32 ± 10.10) N,compared to group C without statistically significant difference (P ＞ 0.05).Conclusions The construction of tissue engineered bone by the heterogeneous de protein cancellous bone,its osteogenesis is close to autogenous bone,but simple heterogeneous de protein cancellous bone osteogenesis is poor.