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Evodiamine inhibits apoptosis of human osteosarcoma MG-63 cells by blocking Wnt/β-catenin signaling / 国际肿瘤学杂志
Journal of International Oncology ; (12): 86-90, 2017.
Article in Chinese | WPRIM | ID: wpr-506016
ABSTRACT
Objective To investigate the effects and mechanism of evodiamine on the proliferation and apoptosis of osteosarcoma MG-63 cells.Methods MG-63 cells were cultured with evodiamine for 24 hours,and the cell proliferation was evaluated by methyl thiazolyl tetrazolium (MTT) assay.Cell cycle arrest,apoptosis and intracellular Ca2+ accumulation were evaluated by flow cytometry.BALB/C mice model of osteosarcoma was established to investigate the tumor inhibitory effect of evodiamine on human osteosarcoma.Wnt/β-catenin signaling protein expressions in osteosarcoma cells were detected by Western blotting.Results As concentration of evodiamine increasing (0.25 μmol/L,0.5 μmol/L,1.0 μmol/L,2.0 μmol/L and 4.0 μmol/L),the inhibition rate of MG-63 ceils increased [(4.18 ± 1.26)%,(15.49 ± 2.26)%,(40.55 ± 6.57)%,(49.87 ±7.69)% and (60.42 ±8.29)%].The difference was statistically significant between 2.0 μmol/L group and the control group (t =-2.66,P < 0.05).MG-63 cells were cultured with 2.0 μmol/L evodiamine for 24 hours,and the apoptotic rate was (64.67 ± 8.63) %,the proportion of S phase cells was (85.33 ± 9.31)%,the fluorescence of Ca2+ was 97.33 ± 21.31.The corresponding data of the control group were (4.94 ± 0.81) %,(43.67 ± 8.92) % and 28.67 ± 8.92,the differences were statistically significant (t =-11.90,P < 0.05;t =-7.22,P < 0.05;t =-6.65,P < 0.05).On mice model,the tumor weight of evodiamine group and the control group was (2.15 ±0.35)g and (4.29 ±0.49)g respectively,the difference was statistically significant (t =7.95,P < 0.05).Comparing with the control group (1.00 ± 0.00),evodiamine decreased the expression of β-catenin protein (0.72 ± 0.36) and increased the expressions of Bax (1.15± 0.27) and Caspase-3 (1.46 ± 0.18) protein,and the differences were statistically significant (t =-3.05,P < 0.05;t =-6.42,P < 0.05;t =-5.85,P < 0.05).Conclusion Evodiamine inhibits proliferation and induces apoptosis of human osteosarcoma MG-63 cells by blocking Wnt/β-catenin signaling.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Journal of International Oncology Year: 2017 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Journal of International Oncology Year: 2017 Type: Article