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Angiopoietin-like protein 3 induces podocytes actin rearrangement via integrin beta 3 / 中华肾脏病杂志
Chinese Journal of Nephrology ; (12): 43-47, 2017.
Article in Chinese | WPRIM | ID: wpr-506163
ABSTRACT
Objective To explore whether Angiopoietin-like protein 3 (ANGPTL3) is involved in podocyte actin rearrangement,and to analyze whether integrin β3 signal pathway is a key in ANGPTL3 inducing actin rearrangement.Methods The cultured podocytes were divided into six groupswild type,ADR treated,ADR+ Dex,MOCK,ANGPTL3-cDNA,miRNA,and AD +miRNA group.(1) We observed actin cytoskeleton using Invitrogen reagents with confocal microscopy;(2) Actin cytoskeleton after blocking β3 on podocytes was;(3) The expression of total FAK and p-FAK was through Western blotting.Results (1) The wild type podocyte's cytoskeleton is arranged orderly.After ADR treatment,podocyte's actin are rearranged and weaken (P < 0.05).There was no significant difference in actin arrangement between knock-down and MOCK group.In ANGPTL3-cDNA group the podocyte actin was also significantly rearranged;on the contrary,in miRNA +ADR group,the actin rearrangement never obviously happened (P < 0.05).(2) Over-expression of ANGPTL3 podocytes blocked integrin β3 did not happen actin rearrangement.(3) The expression of p-FAK significantly increased in over-expression ANGPTL3 podocytes.Conclusion ANGPTL3 is a key in inducing actin rearrangement.Intergrin β3 maybe a central pathway in ANGPTL3's role with podocytes.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Nephrology Year: 2017 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Nephrology Year: 2017 Type: Article